US 11,891,617 C1 (257th)
Modified complex platform of adeno-associated virus with improved rate of expression of loaded genes and reduced genotoxicity
Suk Chul Bae, Cheongju-si (KR); You Soub Lee, Cheongju-si (KR); Xinzi Chi, Cheongju-si (KR); Tae Geun Park, Cheongju-si (KR); and Woo-Jin Kim, Seoul (KR)
Filed by GENECRAFT INC., Cheongju-si (KR)
Assigned to GENECRAFT INC., Cheongju-si (KR)
Supplemental Examination Request No. 96/050,033, Mar. 22, 2024.
Reexamination Certificate for Patent 11,891,617, issued Feb. 6, 2024, Appl. No. 18/190,630, Mar. 27, 2023.
Ex Parte Reexamination Certificate from Reexamination Ordered under 35 U.S.C. 257 issued on Aug. 12, 2025.
Int. Cl. C12N 15/86 (2006.01)
CPC C12N 15/86 (2013.01) [C12N 2750/14143 (2013.01); C12N 2750/14145 (2013.01)]
AS A RESULT OF REEXAMINATION, IT HAS BEEN DETERMINED THAT:
Claims 1 and 4 are determined to be patentable as amended.
Claims 3 and 6-9, dependent on an amended claim, are determined to be patentable.
Claims 2 and 5 were not reexamined.
1. An adeno-associated virus (AAV) complex, comprising a polynucleotide sequence encoding a transgene between a first inverted terminal repeat (ITR) and a second ITR,
wherein the first ITR is not modified and the second ITR is modified,
wherein in the second ITR, all or part of a stem-loop structure, which is formed of rep-binding element (RBE), RBE′, A, A′, B, B′, C, C′, and D regions, is modified,
wherein the modified [ second ] ITR exists as an open-end without forming any of [ ,
wherein the modified second ITR does not form ] the stem region and the loop region of the stem-loop structure [ , and
wherein the modified second ITR does not form a hairpin structure] .
4. The adeno-associated virus complex of claim 1, wherein in the [ modified ] second ITR, all of B, B′, C, C′, and RBE′ regions are deleted, and either A or A′ region, whichever is closer to 3′-end of (+) strand of the transgene or 5′-end of (−) strand of the transgene, is deleted [ , wherein the modified second ITR exists as an open-end] .